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US Biological Life Sciences mouse tff2 elisa kit

( a ) Schematic illustration of <t>TFF2-flox/reporter</t> (Tre-Tom) generation. ( b-d ) Confocal microscopy images showing baseline expression of TFF2 in agaroseinflated lung tissues of ( b ) naïve and ( c, d ) N.b. -infected TFF2-TdTomato fluorescent reporter (Tre-Tom) mice on d4 at ( c ) 200× and ( d ) 400×. ( e, f ) Quantitative Tff2 mRNA levels in sorted ( e ) epithelial cells and ( f ) AM from naïve (open bars) or d4 N.b. -infected (grey bars) lungs of CD11c Cre , CD11c Cre TFF2 flox and TFF2 −/− mice. ( g ) Percentages of BrdU + AT2 cells in CD11c Cre vs CD11c Cre TFF2 flox lungs at indicated time-points after N.b. infection. ( h ) Change of blood oxygen levels from baseline at d3 and d9 following N.b. infection in CD11c Cre and CD11c Cre TFF2 flox mice. ( i ) Representative lung pathology of naïve (left) and d9 following N.b. infection (right) in CD11c Cre and CD11c Cre TFF2 flox mice. Arrowheads indicate emphysematous areas. 20× magnification. *p<0.05, **p<0.01 determined by ANOVA or Student’s t-test. Mean ±SEM are shown.

Mouse Tff2 Elisa Kit, supplied by US Biological Life Sciences, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/mouse+tff2+elisa+kit/pmc06301101-226-0-7?v=US+Biological+Life+Sciences
Average 90 stars, based on 1 article reviews

mouse tff2 elisa kit - by Bioz Stars, 2026-07

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1) Product Images from "Macrophages promote epithelial proliferation following infectious and non-infectious lung injury through a Trefoil factor 2-dependent mechanism"

Article Title: Macrophages promote epithelial proliferation following infectious and non-infectious lung injury through a Trefoil factor 2-dependent mechanism

Journal: Mucosal immunology

doi: 10.1038/s41385-018-0096-2

( a ) Schematic illustration of TFF2-flox/reporter (Tre-Tom) generation. ( b-d ) Confocal microscopy images showing baseline expression of TFF2 in agaroseinflated lung tissues of ( b ) naïve and ( c, d ) N.b. -infected TFF2-TdTomato fluorescent reporter (Tre-Tom) mice on d4 at ( c ) 200× and ( d ) 400×. ( e, f ) Quantitative Tff2 mRNA levels in sorted ( e ) epithelial cells and ( f ) AM from naïve (open bars) or d4 N.b. -infected (grey bars) lungs of CD11c Cre , CD11c Cre TFF2 flox and TFF2 −/− mice. ( g ) Percentages of BrdU + AT2 cells in CD11c Cre vs CD11c Cre TFF2 flox lungs at indicated time-points after N.b. infection. ( h ) Change of blood oxygen levels from baseline at d3 and d9 following N.b. infection in CD11c Cre and CD11c Cre TFF2 flox mice. ( i ) Representative lung pathology of naïve (left) and d9 following N.b. infection (right) in CD11c Cre and CD11c Cre TFF2 flox mice. Arrowheads indicate emphysematous areas. 20× magnification. *p<0.05, **p<0.01 determined by ANOVA or Student’s t-test. Mean ±SEM are shown.

Figure Legend Snippet: ( a ) Schematic illustration of TFF2-flox/reporter (Tre-Tom) generation. ( b-d ) Confocal microscopy images showing baseline expression of TFF2 in agaroseinflated lung tissues of ( b ) naïve and ( c, d ) N.b. -infected TFF2-TdTomato fluorescent reporter (Tre-Tom) mice on d4 at ( c ) 200× and ( d ) 400×. ( e, f ) Quantitative Tff2 mRNA levels in sorted ( e ) epithelial cells and ( f ) AM from naïve (open bars) or d4 N.b. -infected (grey bars) lungs of CD11c Cre , CD11c Cre TFF2 flox and TFF2 −/− mice. ( g ) Percentages of BrdU + AT2 cells in CD11c Cre vs CD11c Cre TFF2 flox lungs at indicated time-points after N.b. infection. ( h ) Change of blood oxygen levels from baseline at d3 and d9 following N.b. infection in CD11c Cre and CD11c Cre TFF2 flox mice. ( i ) Representative lung pathology of naïve (left) and d9 following N.b. infection (right) in CD11c Cre and CD11c Cre TFF2 flox mice. Arrowheads indicate emphysematous areas. 20× magnification. *p<0.05, **p<0.01 determined by ANOVA or Student’s t-test. Mean ±SEM are shown.

Techniques Used: Confocal Microscopy, Expressing, Infection

( a ) BAL TFF2 levels from PBS-treated or at d9 or d16 after Bleomycin (BLM) treatment in CD11c Cre and CD11c Cre -TFF2flox mice. ( b ) Weight changes over two weeks after BLM treatment in CD11c Cre (black symbols) and CD11c Cre TFF2flox (grey symbols) mice. ( c ) Total protein levels in BAL from PBS-treated or d9 BLM-treated mice. ( d ) Percentage of BrdU+EpCAM+ cells in the lungs from PBS controls or on d9 after BLM treatment. Each symbol represents individual mouse. ( e , f ) Message RNA levels of ( e ) SpC and ( f ) Cc10 from lungs of PBStreated or d9/d16 BLM-treated CD11c Cre (open bars) or CD11c Cre -TFF2flox (grey bars) mice. Mean ±SEM are shown. *p<0.05 and **p< 0.01 as determined by ANOVA or Student’s t-test. Representative results from three independent experiments were shown. N=3–6/group unless specified otherwise.

Figure Legend Snippet: ( a ) BAL TFF2 levels from PBS-treated or at d9 or d16 after Bleomycin (BLM) treatment in CD11c Cre and CD11c Cre -TFF2flox mice. ( b ) Weight changes over two weeks after BLM treatment in CD11c Cre (black symbols) and CD11c Cre TFF2flox (grey symbols) mice. ( c ) Total protein levels in BAL from PBS-treated or d9 BLM-treated mice. ( d ) Percentage of BrdU+EpCAM+ cells in the lungs from PBS controls or on d9 after BLM treatment. Each symbol represents individual mouse. ( e , f ) Message RNA levels of ( e ) SpC and ( f ) Cc10 from lungs of PBStreated or d9/d16 BLM-treated CD11c Cre (open bars) or CD11c Cre -TFF2flox (grey bars) mice. Mean ±SEM are shown. *p<0.05 and **p< 0.01 as determined by ANOVA or Student’s t-test. Representative results from three independent experiments were shown. N=3–6/group unless specified otherwise.

Techniques Used:

( a ) Schematic showing MERA conceptual design. ( b ) Trans-epithelial resistance (TER) values of transwell inserts containing primary tracheal epithelia or bone marrow macrophages (BMMφ) cultured alone. ( c ) TER values following scratch wounding of epithelia in the presence or absence of WT BMMφ. ( d,e ) TER values following epithelial injury in the presence of ( d ) WT vs. IL-4Rα −/− BMMφ and ( e ) WT vs. TFF2 −/− BMMφ. ( f ) Levels of TFF2 in MERA cultures with no BMMφ, WT BMMφ, TFF2 −/− BMMφ or IL-4Rα −/− BMMφ. Symbols represent individual wells. ( g ) Gating strategy to identify live epithelial cells recovered from MERA wells that express junctional adhesion molecule (JAM)-1 (R1). ( h-l ) Representative flow plots showing BrdU incorporation in R1 from “ g ” on d4 postscratch wounding ( h ) in the absence of Mφ or in the presence of ( i ) WT BMMφ, ( j ) TFF2 −/− BMMφ, and ( k ) IL-4Rα −/− BMMφ. ( l ) BrdU incorporation on d4 postscratch wounding following treatment with 0.5 ng/ml rTFF2 in the absence of BMMφ. ( m ) Quantification of BrdU + epithelial cells as identified in “ h ” to “ l ”. N=36/group. ( n ) TER values after scratch wounding of epithelia in the presence of rTFF2 (0.5 or 5 ng/ml). ( o ) TER values following scratch wounding of WT vs. TFF2KO epithelia in the presence of WT or TFF2KO alveolar macrophages. ( p, q ) MERA using AT2 co-cultured without BMM or with WT vs. TFF2 −/− BMM. ( p ) Flow plots and ( q ) quantification of EdU + cells. ( r ) TER values following scratch in wells with WT vs. TFF2 −/− BMM. Data represent 2–4 independent experiments. *, p< 0.05; **, p< 0.01 and ***, p< 0.005 as determined by one-way ANOVA or t-test.

Figure Legend Snippet: ( a ) Schematic showing MERA conceptual design. ( b ) Trans-epithelial resistance (TER) values of transwell inserts containing primary tracheal epithelia or bone marrow macrophages (BMMφ) cultured alone. ( c ) TER values following scratch wounding of epithelia in the presence or absence of WT BMMφ. ( d,e ) TER values following epithelial injury in the presence of ( d ) WT vs. IL-4Rα −/− BMMφ and ( e ) WT vs. TFF2 −/− BMMφ. ( f ) Levels of TFF2 in MERA cultures with no BMMφ, WT BMMφ, TFF2 −/− BMMφ or IL-4Rα −/− BMMφ. Symbols represent individual wells. ( g ) Gating strategy to identify live epithelial cells recovered from MERA wells that express junctional adhesion molecule (JAM)-1 (R1). ( h-l ) Representative flow plots showing BrdU incorporation in R1 from “ g ” on d4 postscratch wounding ( h ) in the absence of Mφ or in the presence of ( i ) WT BMMφ, ( j ) TFF2 −/− BMMφ, and ( k ) IL-4Rα −/− BMMφ. ( l ) BrdU incorporation on d4 postscratch wounding following treatment with 0.5 ng/ml rTFF2 in the absence of BMMφ. ( m ) Quantification of BrdU + epithelial cells as identified in “ h ” to “ l ”. N=36/group. ( n ) TER values after scratch wounding of epithelia in the presence of rTFF2 (0.5 or 5 ng/ml). ( o ) TER values following scratch wounding of WT vs. TFF2KO epithelia in the presence of WT or TFF2KO alveolar macrophages. ( p, q ) MERA using AT2 co-cultured without BMM or with WT vs. TFF2 −/− BMM. ( p ) Flow plots and ( q ) quantification of EdU + cells. ( r ) TER values following scratch in wells with WT vs. TFF2 −/− BMM. Data represent 2–4 independent experiments. *, p< 0.05; **, p< 0.01 and ***, p< 0.005 as determined by one-way ANOVA or t-test.

Techniques Used: Cell Culture, BrdU Incorporation Assay

( a,b ) Volcano plots showing results of a cDNA-based screen for Wnt pathway target genes expressed as the fold-difference between ( a ) WT Mφ exposed to damaged or quiescent epithelia and ( b ) between WT Mφ vs. TFF2 −/− Mφ exposed to damaged epithelia. BMMφ were recovered at d4 of MERA for analysis. Each point represents mean of three biological replicates. ( c ) TER values during MERA with WT Mφ in the presence of anti-Wnt4a mAb or control IgG. ( d, e ) Flow-sorted AM, IM and CD103 + DC from WT or TFF2 KO lungs at d4 after infection were analyzed for mRNA levels of ( d ) Wnt4 and ( e ) Wnt16 . ( f ) Representative flow plots and ( g ) quantification of BrdU + SpC + epithelia from distal lung digest cells pre-gated on the live, CD45 − , EpCAM + population for each designated genotype following i.n. administration of rWnt4/16+ R-Spondin 1 cocktail (1μg/mouse, 2 doses) or saline and analyzed by flow cytometry at d4 following N.b. infection (N=5/group). Graphs show Mean±SEM. *, p<0.05; **, p<0.01 and ***, p< 0.005 as determined by ANOVA or Student’s t-test.

Figure Legend Snippet: ( a,b ) Volcano plots showing results of a cDNA-based screen for Wnt pathway target genes expressed as the fold-difference between ( a ) WT Mφ exposed to damaged or quiescent epithelia and ( b ) between WT Mφ vs. TFF2 −/− Mφ exposed to damaged epithelia. BMMφ were recovered at d4 of MERA for analysis. Each point represents mean of three biological replicates. ( c ) TER values during MERA with WT Mφ in the presence of anti-Wnt4a mAb or control IgG. ( d, e ) Flow-sorted AM, IM and CD103 + DC from WT or TFF2 KO lungs at d4 after infection were analyzed for mRNA levels of ( d ) Wnt4 and ( e ) Wnt16 . ( f ) Representative flow plots and ( g ) quantification of BrdU + SpC + epithelia from distal lung digest cells pre-gated on the live, CD45 − , EpCAM + population for each designated genotype following i.n. administration of rWnt4/16+ R-Spondin 1 cocktail (1μg/mouse, 2 doses) or saline and analyzed by flow cytometry at d4 following N.b. infection (N=5/group). Graphs show Mean±SEM. *, p<0.05; **, p<0.01 and ***, p< 0.005 as determined by ANOVA or Student’s t-test.

Techniques Used: Control, Infection, Saline, Flow Cytometry

Image Search Results

Journal: Mucosal immunology

Article Title: Macrophages promote epithelial proliferation following infectious and non-infectious lung injury through a Trefoil factor 2-dependent mechanism

doi: 10.1038/s41385-018-0096-2

Figure Lengend Snippet: ( a ) Schematic illustration of TFF2-flox/reporter (Tre-Tom) generation. ( b-d ) Confocal microscopy images showing baseline expression of TFF2 in agaroseinflated lung tissues of ( b ) naïve and ( c, d ) N.b. -infected TFF2-TdTomato fluorescent reporter (Tre-Tom) mice on d4 at ( c ) 200× and ( d ) 400×. ( e, f ) Quantitative Tff2 mRNA levels in sorted ( e ) epithelial cells and ( f ) AM from naïve (open bars) or d4 N.b. -infected (grey bars) lungs of CD11c Cre , CD11c Cre TFF2 flox and TFF2 −/− mice. ( g ) Percentages of BrdU + AT2 cells in CD11c Cre vs CD11c Cre TFF2 flox lungs at indicated time-points after N.b. infection. ( h ) Change of blood oxygen levels from baseline at d3 and d9 following N.b. infection in CD11c Cre and CD11c Cre TFF2 flox mice. ( i ) Representative lung pathology of naïve (left) and d9 following N.b. infection (right) in CD11c Cre and CD11c Cre TFF2 flox mice. Arrowheads indicate emphysematous areas. 20× magnification. *p<0.05, **p<0.01 determined by ANOVA or Student’s t-test. Mean ±SEM are shown.

Article Snippet: Mouse TFF2 ELISA kit was purchased from United States Biological (Salem, MA).

Techniques: Confocal Microscopy, Expressing, Infection

Journal: Mucosal immunology

Article Title: Macrophages promote epithelial proliferation following infectious and non-infectious lung injury through a Trefoil factor 2-dependent mechanism

doi: 10.1038/s41385-018-0096-2

Figure Lengend Snippet: ( a ) BAL TFF2 levels from PBS-treated or at d9 or d16 after Bleomycin (BLM) treatment in CD11c Cre and CD11c Cre -TFF2flox mice. ( b ) Weight changes over two weeks after BLM treatment in CD11c Cre (black symbols) and CD11c Cre TFF2flox (grey symbols) mice. ( c ) Total protein levels in BAL from PBS-treated or d9 BLM-treated mice. ( d ) Percentage of BrdU+EpCAM+ cells in the lungs from PBS controls or on d9 after BLM treatment. Each symbol represents individual mouse. ( e , f ) Message RNA levels of ( e ) SpC and ( f ) Cc10 from lungs of PBStreated or d9/d16 BLM-treated CD11c Cre (open bars) or CD11c Cre -TFF2flox (grey bars) mice. Mean ±SEM are shown. *p<0.05 and **p< 0.01 as determined by ANOVA or Student’s t-test. Representative results from three independent experiments were shown. N=3–6/group unless specified otherwise.

Article Snippet: Mouse TFF2 ELISA kit was purchased from United States Biological (Salem, MA).

Techniques:

Journal: Mucosal immunology

Article Title: Macrophages promote epithelial proliferation following infectious and non-infectious lung injury through a Trefoil factor 2-dependent mechanism

doi: 10.1038/s41385-018-0096-2

Figure Lengend Snippet: ( a ) Schematic showing MERA conceptual design. ( b ) Trans-epithelial resistance (TER) values of transwell inserts containing primary tracheal epithelia or bone marrow macrophages (BMMφ) cultured alone. ( c ) TER values following scratch wounding of epithelia in the presence or absence of WT BMMφ. ( d,e ) TER values following epithelial injury in the presence of ( d ) WT vs. IL-4Rα −/− BMMφ and ( e ) WT vs. TFF2 −/− BMMφ. ( f ) Levels of TFF2 in MERA cultures with no BMMφ, WT BMMφ, TFF2 −/− BMMφ or IL-4Rα −/− BMMφ. Symbols represent individual wells. ( g ) Gating strategy to identify live epithelial cells recovered from MERA wells that express junctional adhesion molecule (JAM)-1 (R1). ( h-l ) Representative flow plots showing BrdU incorporation in R1 from “ g ” on d4 postscratch wounding ( h ) in the absence of Mφ or in the presence of ( i ) WT BMMφ, ( j ) TFF2 −/− BMMφ, and ( k ) IL-4Rα −/− BMMφ. ( l ) BrdU incorporation on d4 postscratch wounding following treatment with 0.5 ng/ml rTFF2 in the absence of BMMφ. ( m ) Quantification of BrdU + epithelial cells as identified in “ h ” to “ l ”. N=36/group. ( n ) TER values after scratch wounding of epithelia in the presence of rTFF2 (0.5 or 5 ng/ml). ( o ) TER values following scratch wounding of WT vs. TFF2KO epithelia in the presence of WT or TFF2KO alveolar macrophages. ( p, q ) MERA using AT2 co-cultured without BMM or with WT vs. TFF2 −/− BMM. ( p ) Flow plots and ( q ) quantification of EdU + cells. ( r ) TER values following scratch in wells with WT vs. TFF2 −/− BMM. Data represent 2–4 independent experiments. *, p< 0.05; **, p< 0.01 and ***, p< 0.005 as determined by one-way ANOVA or t-test.

Article Snippet: Mouse TFF2 ELISA kit was purchased from United States Biological (Salem, MA).

Techniques: Cell Culture, BrdU Incorporation Assay

Journal: Mucosal immunology

Article Title: Macrophages promote epithelial proliferation following infectious and non-infectious lung injury through a Trefoil factor 2-dependent mechanism

doi: 10.1038/s41385-018-0096-2

Figure Lengend Snippet: ( a,b ) Volcano plots showing results of a cDNA-based screen for Wnt pathway target genes expressed as the fold-difference between ( a ) WT Mφ exposed to damaged or quiescent epithelia and ( b ) between WT Mφ vs. TFF2 −/− Mφ exposed to damaged epithelia. BMMφ were recovered at d4 of MERA for analysis. Each point represents mean of three biological replicates. ( c ) TER values during MERA with WT Mφ in the presence of anti-Wnt4a mAb or control IgG. ( d, e ) Flow-sorted AM, IM and CD103 + DC from WT or TFF2 KO lungs at d4 after infection were analyzed for mRNA levels of ( d ) Wnt4 and ( e ) Wnt16 . ( f ) Representative flow plots and ( g ) quantification of BrdU + SpC + epithelia from distal lung digest cells pre-gated on the live, CD45 − , EpCAM + population for each designated genotype following i.n. administration of rWnt4/16+ R-Spondin 1 cocktail (1μg/mouse, 2 doses) or saline and analyzed by flow cytometry at d4 following N.b. infection (N=5/group). Graphs show Mean±SEM. *, p<0.05; **, p<0.01 and ***, p< 0.005 as determined by ANOVA or Student’s t-test.

Article Snippet: Mouse TFF2 ELISA kit was purchased from United States Biological (Salem, MA).

Techniques: Control, Infection, Saline, Flow Cytometry

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